Journal: Neurochemical Research

Article Title: Dynactin Deficiency in the CNS of Humans with Sporadic ALS and Mice with Genetically Determined Motor Neuron Degeneration

doi: 10.1007/s11064-013-1160-7

Figure Lengend Snippet: TaqMan probes used in the studies

Article Snippet: The immunohistochemical reactions with primary antibodies against DCTN1 (1:100; Santa Cruz Biotech.) and DCTN3 (1:500; Atlas) were performed according to streptavidin–biotin peroxidase method.

Techniques:

Journal: Neurochemical Research

Article Title: Dynactin Deficiency in the CNS of Humans with Sporadic ALS and Mice with Genetically Determined Motor Neuron Degeneration

doi: 10.1007/s11064-013-1160-7

Figure Lengend Snippet: Expression of dynactin DCTN1 and DCTN3 mRNA in the CNS of SALS and control cases. The expression was studied by real-time qPCR, as described in the Material and method section. The results were quantified as the ratio of studied dynactin (DCTN1 or DCTN3) expression to the expression of housekeeping genes (B2M and GusB; ΔCt method). No. 1–5, cases with SALS; a – e , control cases. Black bars motor cortex; striped bars sensory cortex

Article Snippet: The immunohistochemical reactions with primary antibodies against DCTN1 (1:100; Santa Cruz Biotech.) and DCTN3 (1:500; Atlas) were performed according to streptavidin–biotin peroxidase method.

Techniques: Expressing, Control

Journal: Neurochemical Research

Article Title: Dynactin Deficiency in the CNS of Humans with Sporadic ALS and Mice with Genetically Determined Motor Neuron Degeneration

doi: 10.1007/s11064-013-1160-7

Figure Lengend Snippet: Mean dynactin subunits expression in various parts of human CNS

Article Snippet: The immunohistochemical reactions with primary antibodies against DCTN1 (1:100; Santa Cruz Biotech.) and DCTN3 (1:500; Atlas) were performed according to streptavidin–biotin peroxidase method.

Techniques: Expressing, Control

Journal: Neurochemical Research

Article Title: Dynactin Deficiency in the CNS of Humans with Sporadic ALS and Mice with Genetically Determined Motor Neuron Degeneration

doi: 10.1007/s11064-013-1160-7

Figure Lengend Snippet: Expression of dynactin DCTN1 and DCTN3 protein in the CNS of SALS and control cases. The expression was studied by Western blotting, as indicated in the Material and method section. Comparable amounts of protein (20 μg for DCTN1 and 40 μg for DCTN3) from representative control (no D) and SALS (no 4) brains were run in each lane. A; DCTN1: line 1 control motor cortex (optical density: OD 651), line 2 control sensory cortex (OD 847), line 3 SALS motor cortex (OD 439), line 4 SALS sensory cortex (OD 519), line 5 control spinal cord (OD 940), line 6 SALS spinal cord (OD 627). b DCTN3: line 1 control motor cortex (OD 211), line 2 control sensory cortex (OD 238), line 3 SALS motor cortex (OD 210), line 4 SALS sensory cortex (OD 290), line 5 control spinal cord (OD 180), line 6 SALS spinal cord (OD 92)

Article Snippet: The immunohistochemical reactions with primary antibodies against DCTN1 (1:100; Santa Cruz Biotech.) and DCTN3 (1:500; Atlas) were performed according to streptavidin–biotin peroxidase method.

Techniques: Expressing, Control, Western Blot

Journal: Neurochemical Research

Article Title: Dynactin Deficiency in the CNS of Humans with Sporadic ALS and Mice with Genetically Determined Motor Neuron Degeneration

doi: 10.1007/s11064-013-1160-7

Figure Lengend Snippet: Representative immunohistochemistry for dynactin DCTN1 on sections from SALS and control human cases. a Immunopossitive neurons and their processes in SALS motor cortex and visible moderate loss of neuronal cells; b positive immunoreactivity in SALS neurons and axons in the sensory cortex; c moderate immunoexpression in pericaria and axons of the preserved SALS anterior horn motoneurons with features of degeneration; d pronounced immunoreactivity of control neuronal processes and pericaria in the motor cortex; e positive immune reaction of different intensity in control axons and neuronal pericaria in the sensory cortex; f very strong immunoreactivity of processes and motoneuron pericaria in control spinal cord anterior horn. Bars 100 μm each

Article Snippet: The immunohistochemical reactions with primary antibodies against DCTN1 (1:100; Santa Cruz Biotech.) and DCTN3 (1:500; Atlas) were performed according to streptavidin–biotin peroxidase method.

Techniques: Immunohistochemistry, Control

Journal: Neurochemical Research

Article Title: Dynactin Deficiency in the CNS of Humans with Sporadic ALS and Mice with Genetically Determined Motor Neuron Degeneration

doi: 10.1007/s11064-013-1160-7

Figure Lengend Snippet: Representative immunohistochemistry for dynactin DCTN3 on sections from SALS and control human cases. a Weakly immunopositive single neuron in SALS motor cortex ( arrow ) and visible loss of neurons; b mostly negative immune reaction in SALS neurons and axons ( arrow ) in the sensory cortex; c very poor or absent immunoexpression in the preserved motoneurons with features of degeneration in the anterior horn of SALS spinal cord; d weak immunolabel in pericaria of neurons and pronounced in their processes in the control motor cortex; e strong immunoreactivity of axons and poor of neuronal pericaria in the control sensory cortex; f weak immunoexpression in control pericaria of anterior horn motoneurons and mild in axons ( arrows ). Bars 100 μm

Article Snippet: The immunohistochemical reactions with primary antibodies against DCTN1 (1:100; Santa Cruz Biotech.) and DCTN3 (1:500; Atlas) were performed according to streptavidin–biotin peroxidase method.

Techniques: Immunohistochemistry, Control, Immunolabeling

Journal: Neurochemical Research

Article Title: Dynactin Deficiency in the CNS of Humans with Sporadic ALS and Mice with Genetically Determined Motor Neuron Degeneration

doi: 10.1007/s11064-013-1160-7

Figure Lengend Snippet: Expression of dynactin Dctn1 or Dctn3 mRNA in the CNS of transgenic mice. The expression was determined by RT-PCR and expressed as the ratio of the optical density (OD) value of Dctn1 or Dctn3 to the optical density of S12 protein RNA, as indicated in the Material and Method section. Filled diamond dashed lines wild-type controls (+/+); filled triangles Cra1/+ mice; filled circles SOD1/+ mice; filled squares Cra1/SOD1 hybrides

Article Snippet: The immunohistochemical reactions with primary antibodies against DCTN1 (1:100; Santa Cruz Biotech.) and DCTN3 (1:500; Atlas) were performed according to streptavidin–biotin peroxidase method.

Techniques: Expressing, Transgenic Assay, Reverse Transcription Polymerase Chain Reaction

Journal: Neurochemical Research

Article Title: Dynactin Deficiency in the CNS of Humans with Sporadic ALS and Mice with Genetically Determined Motor Neuron Degeneration

doi: 10.1007/s11064-013-1160-7

Figure Lengend Snippet: Percentage of dynactin subunits expression in the CNS of transgenic mice

Article Snippet: The immunohistochemical reactions with primary antibodies against DCTN1 (1:100; Santa Cruz Biotech.) and DCTN3 (1:500; Atlas) were performed according to streptavidin–biotin peroxidase method.

Techniques: Expressing, Transgenic Assay